AZDye™ 647 Picolyl Azide is an advanced fluorescent probe that incorporates a copper-chelating motif to raise the effective concentration of Cu(I) at the reaction site to boost the efficiency of the CuAAC reaction, resulting in a faster and more biocompatible CuAAC labeling. Up to 40-fold increase of signal intensity, compared to conventional azides, was reported (see Selected References).
In addition, the use picolyl azides instead of conventional azides allows for at least a tenfold reduction in the concentration of the copper catalyst without sacrificing the efficiency of labeling, significantly improving biocompatibility of CuAAC labeling protocol.
In summary, the introduction of a copper-chelating motif into azide probe leads to a substantial increase in the sensitivity and reduced cell toxicity of CuAAC detection alkyne-tagged biomolecules. This will be of special value for the detection of low abundance targets or living system imaging.
AZDye™ 647 Picolyl Azide is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines. AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||648/671 nm|
|Flow Cytometry Laser Line|
633 nm or 647 nm
|Microscopy Laser Line|
633 nm or 647 nm
|Spectrally Similar Dyes||Alexa Fluor® 647, CF® 647, DyLight® 649|
|Molecular weight||1061.21 (protonated)|
|Solubility||Water, DMSO, DMF|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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