AZDye™ 647 Alkyne is a water-soluble, pH-insensitive from pH 4 to pH 10, far-red-fluorescent probe with excitation ideally suited for the 633 nm or 647 nm laser lines, and visualized with Cy5 filter sets.
AZDye™ 647 is often a reagent of choice for imaging of low abundance azide-containing biomolecules. AZDye™ 647 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
AZDye™ 647 is structurally similar to Alexa Fluor® 647, and spectrally is almost identical to Cy5 Dye, Alexa Fluor® 647, CF® 647 Dye, or any other Cyanine5 based fluorescent dyes. AZDye™ 647 Azide can be used as a less expensive alternative to Alexa Fluor® 647 Azide.
For application where the presence of copper is not acceptable, please consider our AZDye™ 647 DBCO or Cy5 DBCO probes for copper-less detection of azide-modified molecules.
Alexa Fluor® is a registered trademark of Thermo Fisher Scientific.
Unit Size | 1 mg, 5 mg, 25 mg |
---|---|
Abs/Em Maxima | 648/671 nm |
Extinction Coefficient | 270,000 |
Flow Cytometry Laser Line | 633 nm or 647 nm |
Microscopy Laser Line | 633 nm or 647 nm |
Spectrally Similar Dyes | Alexa Fluor® 647, CF® 647, DyLight® 649 |
Molecular weight | 896.07 (protonated) |
CAS | N/A |
Solubility | Water, DMSO, DMF |
Purity | >95% (HPLC) |
Appearance | Blue solid |
Storage Conditions | -20°C. Desiccate |
Shipping Conditions | Ambient temperature |
Applicable patents and legal notices are available at legal notices.
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