AZDye 594 Biotin (Alexa Fluor® 594 Biotin equivalent) can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. AZDye 594 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||590/617 nm|
|Flow Cytometry Laser Line|
532 or 594 nm
|Microscopy Laser Line|
|Spectrally Similar Dyes||Alexa Fluor® 594, Atto™ 594, CF™ 594 Dye, DyLight™594|
|Solubility||Water, DMSO, DMF, MeOH|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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