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AZDye 594 Biotin (Alexa Fluor® 594 Biotin equivalent) can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. AZDye 594 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 590/617 nm |
| Extinction Coefficient | 88,000 |
| Flow Cytometry Laser Line | 532 or 594 nm |
| Microscopy Laser Line | 594 nm |
| Spectrally Similar Dyes | Alexa Fluor® 594, Atto™ 594, CF™ 594 Dye, DyLight™594 |
| Molecular weight | 1123.32 |
| CAS | N/A |
| Solubility | Water, DMSO, DMF, MeOH |
| Purity | >95% (HPLC) |
| Appearance | Blue solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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