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AZDye™ 555 Alkyne is a water-soluble, bright, orange-fluorescent dye with excitation ideally suited for the 532 nm or 555 nm laser lines and visualized with TRITC (tetramethylrhodamine) filter sets. AZDye™ 555 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker.
AZDye™ 555 is structurally similar to Alexa Fluor® 555, and spectrally is almost identical to Cy3 Dye, Alexa Fluor® 555, CF® 555 Dye, or any other Cyanine3 based fluorescent dyes. AZDye™ 555 Alkyne can be used as less expensive alternative to Alexa Fluor® 555 Alkyne.
For application where the presence of copper is not acceptable, please consider our AZDye™ 555 DBCO or Cy3 DBCO probes for copper-less detection of azide-modified molecules.
Alexa Fluor® 555 is a registered trademark of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 555/572 nm |
| Extinction Coefficient | 155,000 |
| Flow Cytometry Laser Line | 532 nm or 555 nm |
| Microscopy Laser Line | 532 nm or 555 nm |
| Spectrally Similar Dyes | Alexa Fluor® 555, CF® 555, DyLight® 549, Cy3 Dye |
| Molecular weight | 883.06 (protonated) |
| CAS | N/A |
| Solubility | Water, DMSO, DMF |
| Purity | >95% (HPLC) |
| Appearance | Red solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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