AZDye™ 405 NHS Ester is a water-soluble, blue-fluorescent dye that is often used in multi-color applications, including flow cytometry and super-resolution microscopy using STORM. Its excitation is ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 conjugates are pH-insensitive from pH 4 to pH 10.
The NHS ester (or succinimidyl ester) is the most popular amine reactive group for labeling with the primary amines of proteins (Lys), amine-modified oligonucleotides, and other amine-containing molecules. Proteins can be labeled with AZDye™ 405 NHS Ester at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection.
|Unit Size||1 mg, 5 mg, 25 mg, 100 mg|
|Abs/Em Maxima||402/424 nm|
|Extinction coefficient||33,000 cm-1M-1|
|Solubility||Water, DMSO, DMF|
|Spectrally similar dyes||Alexa Fluor® 405, DyLight® 405, Cascade Blue|
|Molecular weight||726.05 (protonated)|
|Shipping Conditions||Ambient temperature|
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