Water-soluble, substrate for sortase mediated labeling of proteins. Sortase catalyzes a transpeptidase reaction between a specific internal sequence of a protein and an amine group present on the N-terminus of triglycine recently has become an area of great interest. This method of labeling proteins has been denoted as “Sortagging”.
AZDye™ 405 is a water-soluble, pH-insensitive from pH 4 to pH 10, blue-fluorescent probe with excitation ideally suited for the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. AZDye™ 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.
| Unit Size | 1 mg, 5 mg, 25 mg |
|---|---|
| Abs/Em Maxima | 405/425nm |
| Extinction Coefficient | 35,000 |
| Spectrally Similar Dyes | Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405 |
| Molecular weight | 889.92 |
| Solubility | Water, DMSO, DMF |
| Appearance | Yellow to grey solid |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Ambient temperature |
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