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AZDye 405 Alkyne (CCT-1309)

[text-backorder]

This product was previously sold as 1067 from Fluoroprobes.

Description

AZDye™ 405 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. AZDye™ 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.

AZDye™ 405 is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.

AZDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405Alexa Fluor® 405CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.

DyLight® and Alexa Fluor® are a registered trademarks of Thermo Fisher Scientific. CF® Dye is a registered trademark of Biotium, Inc

Specifications

Unit Size1 mg, 5 mg, 25 mg
Abs/Em Maxima402/424 nm
Extinction Coefficient
35,000
Flow Cytometry Laser Line
405 nm
Microscopy Laser Line
405 nm
Spectrally Similar DyesAlexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405
Molecular weight553.53 (protonated), 619.47 (sodium salt)
CASN/A
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceYellow solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF405 AF405

Selected References

  1. Costello, A., et al. (2021). Directed evolution of rRNA improves translation kinetics and recombinant protein yield. Nat Commun.12 (1), 5638. [PubMed]

Applicable patents and legal notices are available at legal notices.

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