AZDye™ 405 Alkyne reacts with azides via a copper-catalyzed click reaction (CuAAC) to form a stable triazole linker. AZDye™ 405 is often used in multicolor applications, including flow cytometry and super-resolution microscopy using STORM.
AZDye™ 405 is a bright, blue-fluorescent probe optimally excited by the 407 nm spectral line of the krypton laser or the 408 nm violet laser diode. This probe is water-soluble and its fluorescence is pH independent over a wide pH range. The brightness and photostability of this dye are best suited to direct imaging of moderate-abundance targets.
AZDye™ 405 is structurally similar to Alexa Fluor® 405 or Cascade Blue, and spectrally is a match to DyLight® 405, Alexa Fluor® 405, CF® 405S Dye, Cascade Blue, or any other sulfonated pyrene based fluorescent dyes.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||402/424 nm|
|Flow Cytometry Laser Line|
|Microscopy Laser Line|
|Spectrally Similar Dyes||Alexa Fluor® 405, CF® 405, Cascade Blue®, DyLight® 405|
|Molecular weight||553.53 (protonated), 619.47 (sodium salt)|
|Solubility||Water, DMSO, DMF|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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