DyLight 488 Streptavidin can be used to detect biotinylated secondary antibodies and other macromolecules in applications such as immunofluorescence, in situ hybridization, or flow cytometry.
Features:
Advantages of DyLight® dyes include greater photostability, pH independence, and brighter fluorescence
Excitation maximum: 493 nm
Emission maximum: 518 nm
Color: Green
Specifications
More Information
Unit Size
1 mg
Applications
Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Recommended Usage
Recommended concentration range for use: 5-30 µg/ml. Avoid using RPMI 1640 or other biotin-containing solutions as diluents. Serum also can contain biotin and should not be added to diluents.
Solution
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide.
Vector Laboratories fluorochrome-conjugated streptavidin and avidin reagents are highly purified and possess very low non-specific binding properties. They have extremely high affinity for biotin.
Amplification of fluorescent signals can be easily achieved with our biotinylated secondary antibodies followed by our highly purified fluorochrome-labeled streptavidin or avidin. Using a biotin/avidin or biotin/streptavidin detection system results in an additional layer of amplification over a directly conjugated secondary antibody.
DyLight® dyes offer several advantages including greater photostability, pH independence, and brighter fluorescence. We offer DyLight® conjugated streptavidin for use in a variety of applications, in particular, cell- and tissue-based immunofluorescent detection. The DyLight® conjugates are completely stable from pH 4 to pH 9, making them compatible with many aqueous-based buffers and diluents.
*Additional information about the images can be found here.