Amino-dPEG®₁₂-OH

Amino-dPEG®12-OH, product number QBD-10170, also known as Amino-dPEG®12-alcohol, is a medium-length, monodispersed PEGylation reagent designed to modify surfaces and biomolecules. One end of the molecule terminates with a primary amine, while the other end terminates with a primary alcohol group. This extremely hydrophilic PEGylation reagent modifies biomolecules and surfaces that possess carboxylate groups. If desired, the terminal alcohol moiety can be transformed with appropriate functional groups to further modify the biomolecule or surface.

Amino-dPEG®12-OH has many possible uses. The most common uses are modifying biomolecules and passivating surfaces with a highly hydrophilic coating. The primary amine on one end of the dPEG® linker reacts with carboxylates, aldehydes, and ketones. Conjugations with this molecule most commonly use the amine-carboxylate reaction because it forms stable amide bonds. A carbodiimide such as EDC directly connects the amino group to a carboxylate. Alternatively, activation of carboxylate as the NHS or TFP ester followed by reaction under slightly basic conditions also permits conjugation of the carboxylate and amino moieties.

The amino group also reacts with aldehydes and ketones to form Schiff bases. Schiff bases are reducible to secondary amines for improved stability.

The published uses of Amino-dPEG®12-OH include the following:
Stabilizing artificial membranes;
Real-time imaging of in vivo protease expression;
Developing a confocal imaging system to monitor passive membrane transport; and,
Development of a label-free pyrophosphate detector.