Goat Anti-Rabbit IgG Antibody (H+L)

AMCA Goat Anti-Rabbit IgG Antibody can be used for immunofluorescence and other applications. Optimal F/P ratios have been established for each conjugate to ensure maximum fluorescence with minimal background staining.

Features:

• Recognizes both heavy and light chains (H+L)
• Optimally labeled with AMCA to provide the brightest label for fluorescence microscopy
• Supplied in solution
• Excitation: 350 nm
• Emission: 450 nm
• Color: Blue

$205.00

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SKU: CI-1000-1.5
Color of Fluorescence
Blue
Format
Concentrate
Formulation
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Maximum Emission
448-454 nm.
Maximum Excitation
345-355 nm
Unit Size
1.5 mg
Storage Instructions
2-8 °C
Usage Summary
The recommended concentration range for use is 5-20 µg/ml
Applications
Immunofluorescence, In situ hybridization, Flow Cytometry/Cell Separation
Target Species
Rabbit
Concentration
1.5 mg active conjugate/ml
Conjugate
AMCA
Reactive Species
Goat
Source Species
Rabbit
Host Species
Goat
Technical Information

The goat anti-rabbit Ig antibodies are prepared by hyperimmunizing animals in a manner that produces high affinity antibodies. These are then purified by an affinity chromatography procedure designed to remove any low affinity antibodies which may be present. Cross-reactivities that are likely to interfere with specific labeling are removed by solid-phase adsorption techniques. The final product is then subjected to rigorous quality control assays including immunodiffusion, solid-phase enzyme immunoassays, gel electrophoresis and solid-phase binding assays. In preparing the labeled antibodies, great care is taken to ensure the maximum degree of labeling with no alteration in the specificity and affinity of the antibody. The labeled antibody then undergoes a further series of quality control assays, including immunohistochemical analysis.