5-Ethynyl Uridine (5-EU) (CCT-1261)


5-Ethynyl uridine (5-EU) is an alkyne-containing metabolic labeling reagent used to detect global RNA transcription temporally and spatially in both in-vitro and in-vivo with the modified nucleotide. The alkyne handler can be used for subsequent ligation to azide-containing molecules through a highly efficient click chemistry reaction.

Molecular biology grade 5-Ethynyl Uridine is HPLC purified to achieve the highest purity possible.


Unit Size10 mg, 25 mg, 100 mg, 500 mg
Molecular weight268.22
Chemical compositionC11H12N2O6
SolubilityWater, DMSO, DMF
Purity>95% (HPLC)
AppearanceOff white to grey crystalline
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Selected References

  1. Morral, C., et al. (2020). Zonation of Ribosomal DNA Transcription Defines a Stem Cell Hierarchy in Colorectal Cancer. Cell Stem Cell.26 (6), 845-861.e12. [PubMed]
  2. Banerjee, A.K.,et al. (2020). SARS-CoV-2 disrupts splicing, translation, and protein trafficking to suppress host defenses. Cells., [doi: https://doi.org/10.1016/j.cell.2020.10.004]

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