Multiplexing Solutions
Diverse Options. Reliable Results.
Unlock Deeper Insights with Advanced Multiplexing Solutions
Multiplex immunostaining is revolutionizing spatial biology, personalized medicine, and translational research by enabling researchers to simultaneously detect multiple biomarkers within a single tissue specimen. By preserving the spatial context of biomolecular interactions, multiplexing provides a comprehensive molecular and cellular view offering unparalleled insights into complex cellular relationships, biomarker expression patterns, and disease mechanisms. Scientists across disciplines—from oncology and neuroscience to regenerative medicine—rely on multiplexing to maximize data acquisition from limited samples, accelerate biomarker discovery, and improve diagnostic precision, ultimately deepening our understanding of tissue biology and disease progression.
Vector Laboratories: Pioneering Solutions for Complex Workflows
Vector Laboratories is committed to advancing multiplex immunostaining methodologies. Our extensive portfolio of detection reagents is designed to address the diverse needs of multiplex staining workflows, ensuring researchers have the tools required to achieve clear, reliable, and reproducible results. In addition to our comprehensive portfolio of detection reagents, Vector has recently launched VectaPlex™, our latest innovation designed to eliminate cross-reactivity between multiplex staining rounds. This ready-to-use kit removes antibodies and other non-covalently bound detection reagents from formalin-fixed paraffin-embedded (FFPE) tissue sections in immunohistochemistry, immunofluorescence, and tyramide signal amplification workflows. VectaPlex streamlines the multiplexing process by reducing assay optimization time while preserving tissue integrity and antigenicity of targets, all while delivering precise marker localization.
Figure 1: Stomach section stained with 6 mouse primary antibodies (CD20 (Red), CD34 (Yellow), DES (Cyan), AE1/AE3 (Magenta), CD3 (Grey), VIM (Green)) detected with DyLight™ 488 horse anti-mouse IgG and mounted with VECTASHIELD® PLUS with DAPI. This composite image was generated from 6 rounds of immunofluorescent staining using the VectaPlex Antibody Removal Kit.
Figure 2: VectaPlex preserves tissue antigenicity and morphology more than (A) pressure cooking or (B) microwave treatment. Images of tissue damage as shown by hematoxylin staining before any treatment (top row), after 6 rounds of pressure cooking/microwave (middle row) and by VectaPlex (bottom row). VectaPlex preserves tissue morphology while other methods show extensive tissue damage.
To support diverse workflow requirements, Vector offers a range of products tailored for multiplex applications:
Why Choose Vector Laboratories?
Expertise and Innovation
With decades of experience, we continually innovate to provide cutting-edge tools that meet the evolving needs of the scientific community.
Comprehensive Support
Our team offers unparalleled technical assistance, ensuring optimal application of our products in your research.
Quality Assurance
We adhere to stringent quality control measures, guaranteeing the reliability and consistency of our reagents.
As a leader in immunodetection, Vector Laboratories empowers researchers with advanced multiplex IHC solutions. Our commitment to quality, innovation, and support positions us as the partner of choice for scientists seeking to unravel the complexities of tissue biology.
Explore our comprehensive suite of products and discover how we can enhance your multiplex IHC and IF workflows.
Learn more about
Resources
IHC Multiplexing Resource Guide
Streamline the immunohistochemistry workflow with this step-by-step guide for selecting the best reagents for your project.
Immunofluorescence Resource Guide
Streamline the immunofluorescence process with this step-by-step guide to finding the optimal reagents for your project.
Immunohistochemistry Resource Guide
Step-by-step guide for selecting the best reagents for your immunohistochemistry workflow.
Automated Staining Protocol with VectaPlex
Learn how VectaPlex Antibody Removal Kit enables multiplex staining on the Leica BOND RX Automated Stainer.
