Confocal image of a whole mount E10.5 mouse embryo (mutant for FGF4 and FGF8) in the hindlimb region, showing the mRNA expression of 4 genes: Islet1 (purple), Pax1 (yellow), Twist1 (blue) and Emx2 (green) and DAPI (cyan). Vector TrueVIEW was used after DAPI staining to quench the autofluorescence caused by paraformaldehyde fixation.

Products used: SP-8400-15