VECTASHIELD Antifade Mounting Media

Antifade mounting media optimized for immunofluorescence applications

Choosing an effective mounting medium is especially important for immunofluorescence imaging. Fluorophores are susceptible to photobleaching and fading from the imaging excitation light, handling, and during storage.

Vector Laboratories is a market leader for mounting media that provide excellent signal retention for image acquisition and specimen archiving.

Complete your IF workflow with VECTASHIELD® and VECTASHIELD® HardSet™ Antifade Mounting Media:

  • Inhibit fading and photobleaching of most fluorophores, dyes, fluorescent proteins, and stains
  • Exhibit ideal refractive indices
  • Are ready-to-use (no warming necessary) and easy to use
  • Inhibit photobleaching even after prolonged storage of mounted slides
  • Are available with or without nuclear or cytoskeletal counterstain, in hardening or non-hardening formulations

VECTASHIELD® Antifade Mounting Medium

  • Glycerol-based, aqueous mountant
  • Remains viscous, rather than solidifying
  • After mounting, cover-slipped slides will not dry out, enabling you to review them for weeks without the need for sealing
  • For prolonged storage, seal coverslips with nail polish applied on the coverslip perimeter

VECTASHIELD® HardSet™ Antifade Mounting Medium

  • Aqueous mountant
  • Hardens at room temperature in as little as 20 minutes
  • Provides easy slide handling
  • Eliminates the need to secure the coverslip with nail polish
  • Convenient for use with oil immersion microscopy
  • Available with or without DAPI or TRITC-phalloidin counterstain
This diagram features established applications for our antifade mounting media formats. VECTASHIELD Antifade Mounting Media are widely utilized to protect the inherent fluorescent properties of traditional and contemporary fluorophores in many applications using epifluorescence and confocal microscopy. The versatility of the original VECTASHIELD format solves the demands of labs and core facilities using multiple platforms and fluorescent markers. Furthermore, VECTASHIELD reagents are also recognized as leading media in emerging techniques such as super resolution microscopy (SRM).
VECTASHIELD Mounting Media are the most widely referenced antifade mounting media for immunofluorescence applications. Currently, over 60,000 published references cite using VECTASHIELD Mounting Media and describe compatibility with over 130 fluorophores and fluorescent markers. This data underscores the prominence of VECTASHIELD reagents in this application. The graphic above highlights the most commonly references fluorophores used in combination with VECTASHIELD Antifade Mounting Media.

VECTASHIELD Antifade Mounting Media (IF) - Products

Product Quantity Price
Cat. No. : H-1000, Unit Size: 10 ml
$138.00
Cat. No. : H-1200, Unit Size: 10 ml
$149.00
Cat. No. : H-1300, Unit Size: 10 ml
$158.00
Cat. No. : H-1400, Unit Size: 10 ml
$149.00
Cat. No. : H-1500, Unit Size: 10 ml
$159.00
Cat. No. : H-1600, Unit Size: 10 ml
$158.00