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Fluorophore-Conjugated Secondaries

These affinity-purified, highly specific antibodies are directed against the most commonly used primary antibody target species and are available with a choice of fluorophores.

Original Fluorophore-Conjugated Secondary Antibodies

All of Vector Laboratories’ fluorophore-conjugated secondary antibodies are affinity-purified and highly cross-adsorbed and are available in a concentrated format. Many are also offered in a convenient ready-to-use format (See VectaFluor™ reagents below):

  • Traditional dyes – Fluorescein, Rhodamine, Texas Red®, AMCA, Phycoerythrin
  • Cy®3 and Cy®5 dyes – for bright and stable fluorescence and use in a variety of applications
    • Cy®3 is bright orange with an excitation/emission of 550 nm/570 nm
    • Cy®5 is a far-red dye with an excitation/emission of 649 nm/670 nm; use it in multiplexing protocols or in super-resolution imaging for its photoswitchable properties
  • DyLight® dyes* – for cell- and tissue-based immunofluorescence and a variety of other applications; offer greater photostability, pH independence, and brighter fluorescence than conventional fluorophores

 

VectaFluor™ Ready-to-use Antibody Reagents

VectaFluor™ Antibody Reagents include DyLight® dye-conjugated secondary antibodies as pre-diluted, ready-to-use solutions. Suitable for use with rabbit, mouse, goat, sheep, and bovine IgG primary antibodies, these photostable fluorescence detection reagents allow you to:

  • Reduce optimization requirements
  • Save time
  • Minimize potential dilution errors
  • Obtain greater reproducibility in collaborative efforts across lab environments 
  • Include ready-to-use 2.5% normal horse serum (15 ml) for blocking.

 

VectaFluor™ Duet IF Double Labeling Kits

VectaFluor™ Duet IF Double Labeling Kits allow you to apply two colors in one step. These kits save time and effort in double-labeling immunofluorescence (IF) protocols, which can be long and tedious. The kits are formulated to detect a mouse and a rabbit primary antibody — with green and red fluorescence — in a single step.

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Dorsal root ganglia cells (neurons and satellite glia) – double label. BIII tubulin, DyLight 594 labeled secondary antibody (red). S100, DyLight 488 labeled secondary antibody (green). Mounted in VECTASHIELD HardSet Mounting Medium with DAPI. Image courtesy of Emma East.

Cy Goat X Rabbit Igg Cy Goat X Rabbit Igg Vectorlabs Cy goat x rabbit igg Vectorlabs

Prostate (FFPE) was antigen retrieved with Antigen Unmasking Solution, and stained with Anti-PSA rabbit primary antibody and Cy3-Goat Anti-Rabbit IgG. Mounted in VECTASHIELD HardSet Mounting Medium with DAPI.

B Mabaeae Rmabki B Mabaeae Rmabki Vectorlabs b mabaeae rmabki Vectorlabs

Colon: Mouse Anti-Cytokeratin (AE1/AE3) and Rabbit Anti-Ki67 detected simultaneously with VectaFluor Duet Immunofluorescence Double Labeling Kit, DyLight 488 Anti-Mouse (green)/DyLight 594 Anti-Rabbit (red). Mounted in VECTASHIELD HardSet Mounting Medium.

Resources

Producing immunofluorescence (IF) staining results with high specificity and sensitivity begins with choosing the best reagents. This guide will help you:

  • Learn about the IF workflow and how to optimize each step
  • Quickly identify and choose the most appropriate fluorescent secondary conjugates
  • Select the best anti-fade medium to preserve your fluorescence signal for imaging and archiving

For those wanting additional details on the simple protocol and mode of action of the TrueVIEW Autofluorescence Quenching Kit, this brochure contains clear, concise scematics, comparision data with other types of quenchers, and customer testimonials.