Product Categories
Enzyme Polymer Detection Systems
Integrated antibody and enzyme technology for streamlined IHC and western blot detection
Our enzyme polymer detection system integrates secondary antibodies with a high-density micropolymer array of reporter enzyme. This technique:
- Introduces more enzyme at the site of primary antibody localization
- Yields a stronger signal with lower background
- Simplifies detection into in a single incubation step
We make our proprietary enzyme polymer detection reagents without large dextrans or other macromolecules as a backbone. This novel approach allows a higher density of enzymes to access a target with minimal interference.
We offer IHC polymer detection systems and western blot polymer detection. These products provide:
- Consistent and sensitive detection
- Enhanced signals and diminished background staining (optimum signal:noise ratio)
- Ready-to-use, pre-diluted reagents in convenient dropper bottles
ImmPRESS® Polymer detection
Our unique polymer conjugation chemistry enables significantly more enzyme to be introduced at the antigen target site, resulting in greater signal intensity and sensitivity.
Enzyme conjugated secondary antibody detection
Standard conjugation methods limit the amount of enzyme available for reactivity and hence result in lower signal intensity and substantially lower sensitivity.
Explore Enzyme Polymer Detection Systems
WestVision™ Peroxidase Polymer antibody conjugates bind mouse or rabbit primary antibodies for western blot detection. The high-affinity interaction produces a sharp signal and low background.
ImmPRESS Polymer reagents consist of unique micropolymers of highly active peroxidase or alkaline phosphatase enzyme attached to cross-adsorbed, affinity-purified secondary antibodies. This micropolymer conjugation provides high sensitivity with very low background for IHC tissue staining applications.
Resources
One-step, two-step, amplify: How to choose the right IHC detection system
Enhancing the signal-to-noise ratio is essential for obtaining viable data. Choosing the appropriate immunohistochemistry detection system can help.
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Top Considerations for Selecting Immunohistochemistry (IHC) Detection Reagents
Choosing the appropriate immunohistochemistry detection reagent can be a daunting task. Here are some considerations to guide you on your path to staining success.
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A Simple Guide to Immunohistochemistry Substrate Selection
This blog discusses different factors to consider—enzyme, sensitivity, color, method of visualization, and stability—when selecting the appropriate substrate (and, consequently, color) for your experiment.
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The Immunohistochemistry Resource Guide will help you navigate through the many choices of detection systems, substrates, and ancillary reagents that complete your workflow.
