What is Click Chemistry?
Click Chemistry refers to a class of chemical reactions that are highly specific, fast, and efficient, allowing scientists to two molecules together under simple conditions.
These reactions are broadly applicable across many areas of research, enabling precise labeling, modification, and conjugation of biomolecules. Unlike homobifunctional linkers, which can lead to random or multi-site binding, click chemistry reactions are directional, ensuring that molecules are conjugated at specific sites, preserving their orientation and functionality. Vector offers a comprehensive portfolio of high-quality click chemistry reagents to meet broad application needs.
Vector has a comprehensive
Click Chemistry Toolbox
providing solutions to meet your unique experimental requirements. Vector’s reagents are manufactured for exceptional quality, scalability, and reproducibility, with rigorous quality control ensuring dependable performance across a wide range of experimental applications.
Why Choose Click Chemistry?
Discover the unique advantages that make Click Chemistry an essential tool for bioconjugation reactions.
| Property | Value |
|---|---|
| High Specificity | Highly specific reactions, even in complex biological environments |
| Rapid Reaction Kinetics | Fast, efficient reactions |
| High Yield and Purity | Near-quantitative yields with minimal by-products |
| Mild, Biocompatible Conditions | Compatibility with biological systems with gentle reaction conditions |
| Modularity | Modular components allow incorporation of diverse functional groups into molecules to meet application requirements |
| Scalable Reactions | Scalable reactions with reproducible results at both small and large scales |
| Solubility | Compatible with both aqueous and organic solvents enabling reactions in a wide range of experimental conditions. |
Official Click Chemistry
TOOLBOX
By Vector Labs
Choosing a Click Chemistry Solution:
| Feature | CuAAC Copper-Catalyzed Azide-Alkyne Cycloaddition | SPAAC Strain-Promoted Azide-Alkyne Cycloaddition | Tetrazine-TCO Ligation |
|---|---|---|---|
| Reaction Partners | Azide and terminal alkyne | Azide and DBCO (cyclooctyne) | Tetrazine and trans-cyclooctene (TCO) |
| Reaction Kinetics | Fast | Moderate | Extremely fast |
| Catalyst Requirement | Requires a copper catalyst (Cu(I)). Accelerating copper-chelating ligands improve efficiency & cytotoxicity | No catalyst required (copper-free) | No catalyst required (copper-free) |
| Biocompatibility | Limited | Highly biocompatible, ideal for in vivo applications | Highly biocompatible, ideal for in vivo applications |
| Cytotoxicity | Can be cytotoxic due to copper | Non-toxic (no copper catalyst) | Non-toxic (no copper catalyst) |
| Reaction Yield | High yield and efficiency in vitro | High yields but slower to reach completion than Tetrazine-TCO | High yields, very efficient |
| Reactant Concentrations | Typically requires micromolar Concentrations of both azide and alkyne, along with a copper catalyst, to achieve efficient yields. | Can function at micromolar to millimolar Concentrations, but lower Concentrations slow the reaction rate. | Highly efficient at nanomolar to micromolar Concentrations of both tetrazine and TCO. |
| Key Advantage | Well-established, efficient, and high-yielding in vitro | Copper-free, bioorthogonal and suitable with mild biological conditions | Fastest click reaction, bioorthogonal, ideal for applications requiring rapid kinetics |
| More Info about CuAAC | More Info about SPAAC | More Info about TCO/Tetrazine |
provide complete, ready-to-use solutions for researchers looking to simplify and accelerate their click chemistry experiments:
- Click-&-Go® Enrichment Kits, allowing for the efficient capture of azide- or alkyne-labeled proteins using agarose resins for downstream mass spectrometry or proteomics.
- Click-&-Go® Plus OPP Protein Synthesis Assay, enabling fast and sensitive detection of nascent proteins through fluorescence imaging.
- Click-&-Go® IsoTaG Kits, empowering intact glycopeptide profiling on azide- or alkyne-modified glycopeptides
- Click-&-Go® Fluorescent Imaging Kits, labeling alkyne-labeled molecules with fluorescent dyes.
- Click-&-Go® Reaction Buffer Kits, performing click chemistry reactions in cell-based or biochemical assays.
making it the ideal choice for scientists across drug development, biological research, materials science, and environmental sensing. With its variety of options, click chemistry enables safe and efficient reactions in living systems and synthetic environments alike. Vector’s comprehensive portfolio of click chemistry reagents provides researchers with reliable, high-quality solutions designed to streamline workflows and deliver consistent, reproducible results across a wide range of applications.
Click Chemistry Toolbox
A versatile collection of reagents, probes, and assay kits for biorthogonal labeling, imaging, enrichment, and proteomics.
Get in touch with us at
[email protected]
to take the next step in your bioconjugate journey.
