Format Concentrate | |
Formulation 10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 3 mg/ml bovine serum albumin. | |
Unit Size 1.5 mg | |
Storage Instructions 2-8 °C; Store frozen for long term storage | |
Usage Summary The recommended concentration range for use is 2-10 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue. NOTE: This product is raised in goat and is not the same as that contained in the VECTASTAIN ABC Kits for the detection of mouse IgG which is raised in horse. | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot, ELISAs | |
Target Species Mouse | |
Concentration 1.5 mg active conjugate/ml | |
Conjugate Biotinylated | |
Reactive Species Goat | |
Source Species Mouse | |
Host Species Goat |
Goat Anti-Mouse IgG Antibody (H+L), Biotinylated
The biotinylated goat anti-mouse IgG secondary antibody is used in an avidin-biotin or streptavidin-biotin detection system. These antibodies can be used for tissue and cell staining, ELISAs, and blots.
Features:
• Recognizes both heavy and light chains (H+L)
• Supplied in solution
$206.00
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Technical Information Vector Laboratories affinity-purified antibodies are of unmatched quality. These antibodies are prepared using proprietary immunization schedules that produce high affinity antibodies. The antibodies are then purified by affinity chromatography, and cross-reactivities that are likely to interfere with specific labeling are removed by solid phase adsorption techniques. The biotinylated secondary antibodies are conjugated to ensure the maximum degree of labeling without compromising the specificity or affinity of the antibody. With some exceptions the recommended dilution for most applications is 1:200. |
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Product FAQs
I am performing IHC on tissue sections using a mouse IgG primary antibody. My detection system is a biotinylated anti-mouse IgG secondary antibody, a VECTASTAIN® Elite ABC peroxidase kit (PK-6100), in combination with a DAB substrate kit (SK-4100), and I do not see any staining. How do I know if the end detection reagents are working?
Make a fresh working solution of DAB substrate per instructions. Place a small volume (~1 mL) of this DAB substrate into a clean glass test tube. To this 1 ml aliquot, add one drop (~50 µL) of Reagent B only from the VECTASTAIN® Elite ABC kit. If the HRP enzyme is active and the DAB reacts with it, an immediate color change will be observed. This indicates the end detection reagents are working appropriately.
