Griffonia (Bandeiraea) Simplicifolia Lectin II (GSL II, BSL II), Unconjugated

This lectin is a dimeric glycoprotein composed of two subunits of nearly identical size with each subunit having disulfide-linked chains and a binding site for α- or β-linked N-acetylglucosamine residues. Unlike other N-acetylglucosamine specific lectins, increasing the number of N-acetylglucosamine residues beyond two does not improve affinity. GSL II has been reported to be unique in its ability to recognize exclusively α- or β-linked N-acetylglucosamine residues on the nonreducing terminal of oligosaccharides.

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SKU: L-1210-5
Molecular Weight
113
Extinction Coefficient
1.25
Inhibiting or Eluting Sugar
GlcNAc
Unit Size
5 mg
Storage Instructions
2-8 °C
Sugar Specificity
Terminal GlcNAc-β, Chitin oligomers, and terminal GlcNAc-a
Usage Summary
Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.8% sodium azide can be used. DO NOT VORTEX.
Applications
Glycobiology
Conjugate
Unconjugated
Technical Information

Inhibiting/Eluting Sugar: Chitin Hydrolysate or 200 mM N-acetylglucosamine