CAS Number N/A | |
Molecular Weight 1520.71; single compound | |
Chemical Formula C₆₇H₁₁₇F₄N₃O₂₈S | |
Purity > 98% | |
Unit Size 100mg, 1000mg | |
Solubility Methylene Chloride, DMAC, DMSO, DMF or Acetonitrile. | |
Spacers dPEG® Spacer is 76 atoms and 99.3 Å | |
Storage Instructions -20°C; Always let come to room temperature before opening; be careful to limit exposure to moisture and restore under an inert atmosphere; stock solutions can be prepared with dry solvent and kept for several days (freeze when not in use). dPEG® pegylation compounds are generally hygroscopic and should be treated as such. This will be less noticeable with liquids, but the solids will become tacky and difficult to manipulate, if care is not taken to minimize air exposure. | |
Shipping Instructions Ambient |
Biotin-dPEG®₂₄-TFP ester
Biotin-dPEG®24-TFP ester, product number QBD-10007, contains a biotin-functionalized dPEG®24 spacer that has been activated with a 2,3,5,6-tetrafluorophenyl (TFP) ester. QBD-10007 is part of a family of dPEG®-biotin reagents that provide different lengths of dPEG® between the biotin moiety and the reactive end group. The active esters can be either N-hydroxysuccinimide (NHS) or 2,3,5,6-tetrafluorophenol (TFP). Compared to NHS esters, TFP esters are more reactive towards primary amines and more stable in water or aqueous buffer. The optimum pH for reaction with TFP esters is in the range of 7.5 – 8.5, and for NHS esters the optimum reaction pH range is 6.0 – 7.5. Biotinylation is the process of covalently attaching biotin to a molecule such as a peptide, protein, or nucleic acid, or a surface such as glass or gold. It is widely used in bioconjugation research and product development. Biotin usually is poorly soluble in water, but the amphiphilic dPEG® linker imparts excellent solubility in water or aqueous buffer and in an organic solvent. The 2,3,5,6-tetrafluorophenyl (TFP) moiety of biotin-dPEG®24-TFP ester reacts with free primary amines, such as the amines on the side chain of lysine, forming a stable amide bond. The biotin moiety is used in labeling experiments, supramolecular construction, affinity chromatography, the creation of biotinylated antibodies, enzyme-linked immunosorbent assays (ELISA), pull-down assays, and many other applications. Biotin-dPEG®24-TFP ester does not cause protein aggregation. This permits experiments that require a high degree of biotin labeling without concerns about loss of protein due to aggregation. Dissolution of the product in organic solvent is not required for conjugation, thus providing easier reaction setup and greater reaction reproducibility. The dPEG® linker in QBD-10007 is significantly longer than the linkers in LC-biotin and LC-LC-biotin. Consequently, dPEG®24-biotin provides better access of the biotin to the avidin or streptavidin binding pocket compared to these older, hydrophobic reagents.
Price range: $287.00 through $1,602.00
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