In tissue sections, autofluorescence is the unwanted fluorescence that can make it difficult or impossible to distinguish antigen-specific signal from non-specific background. The novel, patent-pending Vector® TrueVIEW™ Autofluorescence Quenching Kit specifically binds and quenches autofluorescent elements from non-lipofuscin sources, significantly enhancing signal-to-noise in most immunofluorescence assays.
Du, H. et al. (2018) A novel mouse model of hemangiopericytoma due to loss of Tsc2. Human Mol. Gen. (https://doi.org/10.1093/hmg/ddy289)
Su Y, Hou Y, Wang Q, (2018) The enhanced replication of an S-intact PEDV during coinfection with an S1 NTD-del PEDV in piglets, Veterinary Microbiology (https://doi.org/10.1016/j.vetmic.2018.11.025)
Nishimura, A. et al. (2018) Hypoxia-induced interaction of filamin with Drp1 causes mitochondrial hyperfission–associated myocardial senescence. Science Signaling (https://stke.sciencemag.org/content/11/556/eaat5185.long)
Yoon, J.H., Li, M., Basile, J.R., Lin, Y (2018) Computer-assisted analysis of immunohistological parameters in oral giant cell granulomas, Oral Diseases (https://doi.org/10.1111/odi.13022)
Rodgers, H.M. et al. (2019) Dopamine D1 and D3 receptor modulators restore morphine analgesia and prevent opioid preference in a model of neuropathic pain. Neuroscience (https://www.sciencedirect.com/science/article/abs/pii/S0306452219301927)
Chafe, S.C. et al. (2019) Targeting hypoxia-induced carbonic anhydrase IX enhances immune-checkpoint blockade locally and systemically. Cancer Immunol Res (http://cancerimmunolres.aacrjournals.org/content/early/2019/05/31/2326-6066.CIR-18-0657)
Wilson, M.R. et al. (2019) ARID1A and PI3-kinase pathway mutations in the endometrium drive epithelial transdifferentiation and collective invasion. Nature Communications (https://www.nature.com/articles/s41467-019-11403-6)
Lecocq, Q. et al. (2019) Noninvasive Imaging of the Immune Checkpoint LAG-3 Using Nanobodies, from Development to Pre-Clinical Use. Biomolecules (https://www.mdpi.com/2218-273X/9/10/548/htm)
Whereas most methods for reducing tissue autofluorescence act primarily on lipofuscin granules, the Vector® TrueVIEW™ quencher targets fluorescence from non-lipofuscin sources, including aldehyde fixation, red blood cells, and structural elements, such as collagen and elastin. It provides a clear, unambiguous “true view” of target antigen localization, even in problematic tissues, such as kidney, spleen and pancreas.
Comparisons with other commercials and “home brew” approaches show that Vector® TrueVIEW™ is easier to use and more effective. The images below show the results of side-by-side comparisons on serial sections of formalin-fixed, paraffin embedded human pancreas visualized using a standard fluorescein (green) filter. No specific immunofluorescence staining was conducted.
Listen to the podcast
LISTEN to the podcast by Timothy Karpishin, PhD, Director of Chemistry at Vector Laboratories, Inc. describing TrueVIEW™ Autofluorescence Quenching Kit at the 43rd Annual NSH Symposium/Convention.
Download the poster
DOWNLOAD the poster describing TrueVIEW™ Autofluorescence Quenching Kit presented at the 43rd Annual NSH Symposium/Convention.
Download the brochure for more information
Learn more about the Vector® TrueVIEW™ Autofluorescence Quenching Kit by downloading the brochure.
Following completion of immunofluorescence staining:
|Vector® TrueVIEW™ Autofluorescence Quenching Kit||SP-8400|
|VECTASHIELD® Vibrance™ Antifade Mounting Medium||H-1700|
|VECTASHIELD® Vibrance™ with DAPI Antifade Mounting Medium||H-1800|