Vector Blue Substrate Kit, Alkaline Phosphatase (AP)

The Vector Blue AP Substrate Kit produces a blue, heat stable reaction product. This chromogen can be used singly or in combination with other alkaline phosphatase or peroxidase substrates for multiple label applications.

Features:

• Greater sensitivity than conventional substrates
• Consistent and reliable
• Ideal for IHC/ICC/ISH and blots
• Fluorescent
• Heat Stable
• Permanent mounting
• Stock solutions supplied in convenient dropper bottles promoting ease of handling
• No wait times for mixing and dissolving powders or tablets
• Sufficient reagents to produce 200 ml of working solution
• Excitation: broad, peak at ~500 nm*
• Emission: broad, peak at ~680 nm**Histochem J. (1998) 8:577

$210.00

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SKU: SK-5300
Microscopy
Brightfield, Fluorescence, Spectral Imaging
Unit Size
1 Kit
Applications
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Additional Substrate Properties
Contrast with Tissue Pigments, Fluorescent, Heat Resistant, Multiple Labeling
Kit Components
  • 4 ml Vector Blue Reagent 1
  • 4 ml Vector Blue Reagent 2
  • 3 ml Vector Blue Reagent 3
Technical Information

Vector Blue is both chromogenic and fluorescent. Sections stained with the Vector Blue substrate can be dehydrated, cleared, and permanently mounted; however, non-xylene clearing agents and non-xylene based mounting media (such as VectaMount®, H-5000) must be used because the reaction product is soluble in xylene. Vector Blue can also be used on blots in applications such as ELISPOT, singly or in combination with a peroxidase substrate such as AEC. This kit contains stock solutions in convenient dropper bottles.

Substratesensitivity2 Jpg Substrate.counterstainchart Jpg

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