Molecular Weight 66 | |
Extinction Coefficient 1.5 | |
Inhibiting or Eluting Sugar Galactose | |
Unit Size 25 mg | |
Storage Instructions 2-8 °C | |
Sugar Specificity Core 1 and core 3 O-glycans | |
Usage Summary Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2is recommended.For preserving solutions, stored at 4 ºC, 0.08% sodium azide can be used. | |
Applications Glycobiology, Mitogenic Stimulation | |
Conjugate Unconjugated |
Jacalin, Unconjugated
This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.
$366.00
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Technical Information Jacalin is a lectin composed of four subunits of approximately 16 kDa each. This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains. Inhibiting/Eluting Sugar: 800 mM galactose or 100 mM melibiose |
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