Jacalin, Unconjugated

This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.

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SKU: L-1150-25
Molecular Weight
66
Extinction Coefficient
1.5
Inhibiting or Eluting Sugar
Galactose
Unit Size
25 mg
Storage Instructions
2-8 °C
Sugar Specificity
Core 1 and core 3 O-glycans
Usage Summary
Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2is recommended.For preserving solutions, stored at 4 ºC, 0.08% sodium azide can be used.
Applications
Glycobiology, Mitogenic Stimulation
Conjugate
Unconjugated
Technical Information

Jacalin is a lectin composed of four subunits of approximately 16 kDa each. This lectin appears to bind only O-glycosidically linked oligosaccharides, preferring the structure galactosyl (β-1,3) N-acetylgalactosamine. This structure (the T-antigen) is the oligosaccharide to which peanut agglutinin (PNA) binds. However, unlike PNA, Jacalin will bind a mono- or disialylated form of this structure. This lectin has been used to purify human IgA. The specificity of this lectin also affords the opportunity to localize or isolate glycoproteins with O-glycosidically linked oligosaccharide side chains.

Inhibiting/Eluting Sugar: 800 mM galactose or 100 mM melibiose