The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.
Unit Size | 5 mg |
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Applications | Glycobiology, Mitogenic Stimulation |
Recommended Usage | Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2 is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used. |
Recommended Storage | 2-8 °C |
Conjugate | Unconjugated |
Sugar Specificity | N-Acetylgalactosamine |
Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine
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