Pisum Sativum Agglutinin (PSA), Unconjugated

Pisum sativum agglutinin is nearly identical in structure and carbohydrate specificity to Lens culinaris agglutinin. The lectin has specificity toward α-linked mannose-containing oligosaccharides, with an N-acetylchitobiose-linked α-fucose residue included in the receptor sequence. Calcium and manganese ions are required for activity.

$219.00

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SKU: L-1050-10
Molecular Weight
53
Extinction Coefficient
1.2
Inhibiting or Eluting Sugar
Mixture of a-methyl-mannoside & a-methyl-glucoside
Unit Size
10 mg
Storage Instructions
2-8 °C
Sugar Specificity
α1,6-linked fucose
Usage Summary
Although many buffers can be employed for reconstituting and diluting this lectin, 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2is recommended. For preserving solutions stored at 4 ºC, 0.08% sodium azide can be used.
Applications
Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology, Mitogenic Stimulation
Conjugate
Unconjugated
Technical Information

PSA has been used to fractionate cells, to isolate glycoproteins and glycopeptides, to distinguish between normal and virally transformed cells, as a T-cell mitogen, and as an inhibitor of allograft rejection.

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside