Blocking Action Endogenous Biotin or Avidin/Streptavidin Binding Proteins | |
Format Ready-to-Use | |
Unit Size 1 kit | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot |
Streptavidin/Biotin Blocking Kit
Streptavidin/Biotin Blocking Kit blocks all endogenous biotin, biotin receptors, and streptavidin binding sites present in tissues. This kit is designed for use with streptavidin detection systems such as the VECTASTAIN® Universal Quick kits if streptavidin or biotinylated reagents bind non-specifically to tissues or proteins. This blocking kit consists of 18 ml Streptavidin and 18 ml of biotin in convenient dropper bottles.
$188.00
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Product FAQs
What is the difference between the Avidin/Biotin Blocking Kit, Cat. No. SP-2001, and the Streptavidin/Biotin Blocking Kit, Cat. No. SP-2002, and can they be used interchangeably?
These kits were developed to prevent non-specific binding of avidin or streptavidin based detection reagents primarily to endogenous biotin in cell and tissue IHC and IF applications. It is generally recommended to match the blocking and detection reagent such as using an avidin/biotin blocking kit with an avidin based detection system. In most cases, however, these kits can be used interchangeably, except in circumstances where avidin or streptavidin may bind to inherent non-biotin associated structures (see: Alon, R., et al {1992}, Eur. J. Cell Biol. 58:271-279).
