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Streptavidin/Biotin Blocking Kit (SP-2002)

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Description

Streptavidin/Biotin Blocking Kit blocks all endogenous biotin, biotin receptors, and streptavidin binding sites present in tissues. This kit is designed for use with streptavidin detection systems such as the VECTASTAIN® Universal Quick kits if streptavidin or biotinylated reagents bind non-specifically to tissues or proteins. This blocking kit consists of 18 ml Streptavidin and 18 ml of biotin in convenient dropper bottles.

Specifications

More Information
Unit Size1 kit
ApplicationsImmunohistochemistry / Immunocytochemistry, Immunofluorescence, In situ hybridization, Blotting Applications, Elispot
Blocking ActionEndogenous Biotin or Avidin/Streptavidin Binding Proteins
FormatReady-to-Use

Product FAQs

These kits were developed to prevent non-specific binding of avidin or streptavidin based detection reagents primarily to endogenous biotin in cell and tissue IHC and IF applications. It is generally recommended to match the blocking and detection reagent such as using an avidin/biotin blocking kit with an avidin based detection system. In most cases, however, these kits can be used interchangeably, except in circumstances where avidin or streptavidin may bind to inherent non-biotin associated structures (see: Alon, R., et al {1992}, Eur. J. Cell Biol. 58:271-279).

Citations

Applicable patents and legal notices are available at legal notices.

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