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Ulex Europaeus Agglutinin I (UEA I), Rhodamine (RL-1062-2)

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Description

Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.

Rhodamine labeled Ulex europaeus agglutinin I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.

Specifications

Unit Size2 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8 °C
Maximum Excitation545-555 nm
Maximum Emission570-580 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide
Concentration2 mg active conjugate/ml
ConjugateRhodamine
Color of FluorescenceRed
Sugar SpecificityFucose, Arabinose

Citations

Technical Information

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose

Applicable patents and legal notices are available at legal notices.

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