This lectin consists of two subunits of 60 kDa which can be dissociated by reducing agents into closely related chains between 27 kDa and 33 kDa. One of the chains appears to be common to the “B” chain of another castor bean lectin, ricin, while the other chain is unique to RCA I. The B chain binds to galactose or N-acetylgalactosamine residues of membrane glycoconjugates.
Rhodamine labeled Ricinus communis agglutinin I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 550 nm and the emission maximum is at 575 nm.
|Unit Size||5 mg|
|Recommended Usage||For most applications we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.|
|Recommended Storage||2-8 °C|
|Maximum Excitation||545-555 nm|
|Inhibiting and/or Eluting Sugar||200 mM galactose (S-9003) or lactose (S-9004)|
|Maximum Emission||570-580 nm|
|Solution||10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 5 mM lactose|
|Concentration||5 mg active conjugate/ml|
|Color of Fluorescence||Red|
|Sugar Specificity||Galactose, Lactose|
Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.
Inhibiting/Eluting Sugar: 200 mM galactose or lactose
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