PHA-L, with four L type subunits, does not agglutinate red cells but is a potent mitogen. The other three isolectins, designated E3L1, E2L2, and E1L3, have erythroagglutinating and mitogenic activities proportional to the number of respective E or L subunits. We have termed the mixture of the five isolectins PHA (E+L).
After iontophoretic injection of unconjugated PHA-L, the approximate rate of anterograde transport is about 4-6 mm/day, with survival periods of over 18 days having been observed. Once transported, the unconjugated PHA-L is best visualized for light microscopy with antibody to the lectin, followed by the appropriate detection system and substrate. Fluorescent staining can also be used for a faster staining procedure. A complete applications protocol is available upon request.
Elution: 100 mM acetic acid