Molecular Weight 114 | |
Extinction Coefficient 1.4 | |
Inhibiting or Eluting Sugar Raffinose | |
Unit Size 5 mg | |
Storage Instructions 2-8 °C | |
Sugar Specificity Terminal αGal and terminal αGalNAc | |
Usage Summary Although many buffers can be employed for reconstituting and diluting this lectin, we recommend 10 mM HEPES buffered saline, pH 8.5, 0.1 mM CaCl2.For preserving solutions stored at 4 ºC, add 0.08% sodium azide to the buffer. For dissolving the lectin, we recommend adding the lectin slowly to buffer rather than adding the buffer to the lectin.Allow the lectin to dissolve without vigorous agitation. | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Glycobiology | |
Conjugate Unconjugated |
Griffonia (Bandeiraea) Simplicifolia Lectin I (GSL I, BSL I), Unconjugated
GSL I is a family of glycoproteins with molecular weights of approximately 114 kDa. There are two types of subunits, termed “A” and “B”, with slightly different molecular weights. These subunits combine to form tetrameric structures, resulting in five isolectins. The “A”-rich lectin preferentially agglutinates blood group A erythrocytes and thus appears to be specific for α-N-acetylgalactosamine residues, while the “B”-rich lectin preferentially agglutinates blood group B cells and is specific for α-galactose residues. Our GSL I is a mixture of the five isolectins. GSL I has been reported to bind several glycoproteins including laminin.
$213.00
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Technical Information Inhibiting/Eluting Sugar: mixture of 200 mM galactose/200 mM N-acetylgalactosamine |
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