Molecular Weight 116 | |
Color of Fluorescence Green | |
Extinction Coefficient 0.89 | |
Formulation 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM CaCl2 | |
Inhibiting or Eluting Sugar GalNAc | |
Maximum Emission 514-521 nm | |
Maximum Excitation 495-500 nm | |
Unit Size 2 mg | |
Storage Instructions 2-8°C | |
Sugar Specificity Terminal β-GalNAc, terminal a-GalNAc and terminal multiantennary LacNAc | |
Usage Summary The recommended concentration range for use is 5-20 µg/ml. | |
Applications Immunofluorescence, Glycobiology | |
Concentration 2 mg active conjugate/ml | |
Conjugate Fluorescein |
Wisteria Floribunda Lectin (WFA, WFL), Fluorescein
The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.
Fluorescein labeled WFL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.
• Excitation maximum: 495 nm
• Emission maximum: 515 nm
• Color: Green
$227.00
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Technical Information Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine |
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