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Wisteria Floribunda Lectin (WFA, WFL), Fluorescein (FL-1351-2)

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Description

The binding specificity of Wisteria floribunda lectin (WFL) is not completely clear but this lectin appears to preferentially bind carbohydrate structures terminating in N-acetylgalactosamine linked α or β to the 3 or 6 position of galactose. This lectin has been used to fractionate lymphocyte populations, and although not mitogenic, elicits the production of lymphokines from murine splenocytes.

Fluorescein labeled WFL has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 495 nm
  • Emission maximum: 515 nm
  • Color: Green

 

Specifications

Unit Size2 mg
ApplicationsImmunofluorescence, Glycobiology
Recommended UsageThe recommended concentration range for use is 5-20 µg/ml.
Recommended Storage2-8°C
Maximum Excitation495-500 nm
Inhibiting and/or Eluting Sugar200 mM N-acetylgalactosamine(S-9001)
Maximum Emission514-521 nm
Solution10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM CaCl2
Concentration2 mg active conjugate/ml
ConjugateFluorescein
Color of FluorescenceGreen
Sugar SpecificityN-Acetylgalactosamine

Citations

Technical Information

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 200 mM N-acetylgalactosamine

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