Ulex Europaeus Agglutinin I (UEA I), Fluorescein

Ulex europaeus agglutinin I binds to many glycoproteins and glycolipids containing α-linked fucose residues, such as ABO blood group glycoconjugates. This lectin preferentially binds blood group O cells and has been used to determine secretor status. It has been established as an excellent marker for human endothelial cells.

Fluorescein labeled Ulex europaeus agglutinin I has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

• Excitation maximum: 495 nm
• Emission maximum: 515 nm
• Color: Green

Price range: $253.00 through $400.00

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SKU: FL-1061
Molecular Weight
63
Color of Fluorescence
Green
Extinction Coefficient
1.3
Formulation
10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2
Inhibiting or Eluting Sugar
L-Fucose
Maximum Emission
514-521 nm
Maximum Excitation
495-500 nm
Unit Size
2 mg, 5 mg
Storage Instructions
2-8 °C
Sugar Specificity
α1,2-linked fucose
Usage Summary
The recommended concentration range for use is 5-20 µg/ml.
Applications
Immunofluorescence
Concentration
FL-1061-2 (2 mg active conjugate/ml)FL-1061-5 (5 mg active conjugate/ml)
Conjugate
Fluorescein
Technical Information

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide.

Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose