Maackia amurensis lectin I binds gal (β-1,4) glcNAc but tolerates substitution of N-acetyllactosamine with sialic acid at the 3 position of galactose. However, MAL I does not appear to bind this structure when substitution with sialic acid is on the 6 position of galactose.
Fluorescein labeled Maackia amurensis lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes. The excitation maximum is at 495 nm and the emission maximum is at 515 nm.
|Unit Size||2 mg|
|Recommended Usage||The recommended concentration range for use is 5-20 µg/ml.|
|Maximum Excitation||495-500 nm|
|Inhibiting and/or Eluting Sugar||200 mM lactose (S-9004)|
|Maximum Emission||514-521 nm|
|Solution||10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide. 0.1 mM CaCl2|
|Concentration||2 mg active conjugate/ml|
|Color of Fluorescence||Green|
|Sugar Specificity||Galactose, Lactose|
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