Molecular Weight 71 | |
Color of Fluorescence Green | |
Extinction Coefficient 0.76 | |
Formulation 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, 5mg/ml β cyclodextrin | |
Inhibiting or Eluting Sugar Chitin Hydrolysate | |
Maximum Emission 514-521 nm | |
Maximum Excitation 495-500 nm | |
Unit Size 1 mg | |
Storage Instructions 2-8°C | |
Sugar Specificity Chitin oligomers, type 2 polyLacNAc, and
Type 2 LacdiNAc | |
Usage Summary The recommended concentration range for use is 5-20 µg/ml. | |
Applications Immunofluorescence, Glycobiology | |
Concentration 2 mg active conjugate/ml | |
Conjugate Fluorescein |
Lycopersicon Esculentum (Tomato) Lectin (LEL, TL), Fluorescein
Tomato lectin (from Lycopersicon esculentum) is an effective marker of blood vessels and microglial cells in rodents. Conjugation of the lectin with fluorescein facilitates fast, one-step detection and visualization using intravascular perfusion methods or direct application to tissue sections.
$212.00
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Technical Information Tomato lectin is a very stable single subunit glycoprotein containing about 50 percent arabinose and galactose and may form multimeric aggregates in solution. Tomato lectin, although sharing some specificities with potato lectin, Datura lectin, and wheat germ agglutinin, has been reported to be dissimilar in many respects. LEL binds well to glycophorin and Tamm-Horsfall glycoprotein and has been used effectively to label vascular endothelium in rodents. Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. Fluorescein labeled Tomato lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.
Inhibiting/Eluting Sugar: Chitin Hydrolysate |
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Product FAQs
Can the biotinylated Lycopersicon esculentum (tomato) lectin be used for in vivo perfusion studies to trace blood vessels in mice?
The Lycopersicon esculentum (tomato) lectin has been widely reported as an effective blood vessel marker for in vivo vascular perfusion studies in rodent species. Investigators have primarily utilized one of the fluorophore conjugated tomato lectin formats to trace blood vasculature in normal and diseased animals via tail vein or intracardiac injection. However, the biotinylated format has also been used. It allows for flexibility in subsequent visualization by way of either fluorescence or enzyme-based methods. Published references are best source for procedural details. Examples of references where biotinylated tomato lectins have been applied via in vivo perfusion are featured below: Robertson, R.T., et al. (2014) Histochem. Cell Biol. 143(2) Thurston, G., et al. (1998) Am. J Pathol. 153(4):1099-1112.
