Lens Culinaris Agglutinin (LCA), DyLight™ 649

DL-1048-1
SKU Unit Size Price Qty
DL-1048-1 1 mg
$166.00
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Description

Lens culinaris agglutinin (LCA) recognizes sequences containing α-linked mannose residues but recognizes additional sugars as part of the receptor structure, giving it a narrower specificity than Con A. An α-linked fucose residue attached to the N-acetylchitobiose portion of the core oligosaccharide significantly enhances affinity. By exploiting this increased specificity, glycoproteins and glycopeptides can be subfractionated with LCA after initial isolation with Con A.

DyLight 649 labeled LCA has an appropriate number of bound fluorochromes, which provides the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.

  • Excitation maximum: 655 nm
  • Emission maximum: 670 nm
  • Color: Far red

Lens Culinaris Agglutinin (LCA), DyLight™ 649 is provided in a 1 mL aliquot at a concentration of 1 mg active conjugate/ml.

Specifications

More Information
Unit Size 1 mg
Applications Immunofluorescence, Glycobiology
Recommended Usage The recommended concentration range for use is 5-20 µg/ml. If a precipitate forms upon long-term storage, warm to 37 ºC.
Recommended Storage 2-8 °C
Maximum Excitation 655 nm
Maximum Emission 670 nm
Solution 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2 , 0.01 mM MnCl2
Concentration 1 mg active conjugate/ml
Conjugate DyLight 649
Color of Fluorescence Far Red
Sugar Specificity Mannose, Glucose

Documents

Citations

Technical Information

Lens culinaris agglutinin is composed of four subunits - two of about 17 kDa and two of 8 kDa. 

LCA has been found to be one of the most effective agents in preventing skin allograft rejection in model systems. LCA has been used to purify numerous glycoproteins, including immunoglobulins, histocompatibility antigens, and α2-macroglobulin.

Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. 

Inhibiting/Eluting Sugar: mixture of 200 mM α-methylmannoside/200 mM α-methylglucoside

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