Digoxigenin (DIG) is a small plant-derived molecule not found in animals. DIG is used to label nucleic acid probes for applications such as in situ hybridization. DIG-labeled probes are detected with antibodies specifically directed against the DIG label. We have generated high affinity and highly purified antibodies for this purpose. The DyLight™ conjugated antibodies allow a one-step fluorescent visualization of DIG-labeled probes.
|Unit Size||0.5 mg|
|Applications||In situ hybridization|
|Concentration||1.0 mg active conjugate/ml|
|Recommended Storage||2-8 °C|
|Solution||10 mM HEPES, 0.15 M NaCl, pH 7.8, 0.08% sodium azide.|
|Maximum Emission||518 nm|
|Maximum Excitation||493 nm|
|Recommended Usage||The recommended concentration range for use is 5-20 µg/ml.|
|Detection Target||Digoxigenin/Digoxin (DIG)|
|Color of Fluorescence||Green|
DyLight™ dyes offer several advantages including greater photostability, pH independence and brighter fluorescence. DyLight™ conjugates are completely stable from pH 4 to pH 9, making them compatible with many buffers and diluents.
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