Desthiobiotin is a single-ring, sulfur-free analog of biotin that binds to streptavidin with nearly equal specificity but less affinity than biotin (Kd = 1011 vs. 1015 M, respectively). Consequently, desthiobiotinylated proteins can be eluted readily and specifically from streptavidin affinity resin using mild conditions based on competitive displacement with free biotin. For pull-down experiments with biological samples, this soft-release characteristic of desthiobiotin also helps to minimize co-purification of endogenous biotinylated molecules, which remain bound to streptavidin upon elution of the target protein complexes with free biotin.
| Unit Size | 10 mg, 25 mg, 100 mg |
|---|---|
| Molecular weight | 719.87 |
| Chemical composition | C39H51N5O8 |
| CAS | N/A |
| Solubility | DMSO, DMF, DCM, THF, Chloroform |
| Purity | >95% (HPLC) |
| Appearance | Colorless to slightly yellow oil |
| Storage Conditions | -20°C. Desiccate |
| Shipping Conditions | Frozen |
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