CAS Number N/A | |
Molecular Weight 761.28 (protonated) | |
Appearance Blue solid | |
Extinction Coefficient 270,000 | |
Unit Size 150 slides, 1500 slides | |
Solubility Water, DMSO, DMF | |
Storage Instructions -20°C. Desiccate | |
Spectrally Similar Dyes Alexa Fluor® 647, CF® 647, DyLight® 649 | |
Laser Line 633 nm or 647 nm | |
Excitation/Emission Maximum 648/671 nm | |
Shipping Conditions Ambient temperature | |
Shipping Instructions Ambient temperature |
Cy5 Tyramide
Far-red fluorescent reagent widely used for tyramide signal amplification (TSA) in IHC, ICC, FISH and multicolor FISH. Tyramide signal amplification (TSA) has proven to be a particularly versatile and powerful enzyme amplification technique with improved assay sensitivity. TSA is based on the ability of HRP, in the presence of low concentrations of hydrogen peroxide, to convert labeled tyramine-containing substrate into an oxidized, highly reactive free radical that can covalently bind to tyrosine residues at or near the HRP.
Cy5 Tyramide is a component of many tyramide signal amplification kits. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).
Price range: $180.00 through $711.00
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Product FAQs
What is the quantity of tyramide reagent provided in the 150 slides and 1500 slides unit sizes?
150 slides has 0.24 µmol (depending on molecular weight this is ~200 µg)
1500 slides has 2.4 µmol (depending on molecular weight this is ~2 mg)
What is the protocol for using a Fluorophore Tyramide product in tyramide signal amplification (TSA)?
Here is a general protocol for the 150 slide unit size. To prepare 100x stock solution, we recommend reconstituting with 150 ul of DMSO. Storage once in solution: 100X stock solution in DMSO can be stored for 6 months at 2-8 °C protected from light. Avoid moisture.
Working solutions can be made fresh by adding 1 part 100X stock solution, 1 part 0.15% H202, and 100 part buffer.
For example, you can add 5 µL of 100X Tyramide stock solution and 5 µL 0.15% H2O2 to 500 µL of buffer. The final concentration of H2O2 is 0.0015%.
Typical reaction time following the HRP reagent is 2-10 minutes but should be optimized for each workflow. Following the TSA working solution incubation, wash thoroughly.
