Molecular Weight 140 | |
Color of Fluorescence Far Red | |
Extinction Coefficient 1.5 | |
Formulation 10 mM HEPES, 0.15 M NaCl, pH 7.5, 0.08% sodium azide, 0.1 mM CaCl2, and a proprietary stabilizer | |
Inhibiting or Eluting Sugar Lactose or Neuraminidase treatment | |
Maximum Emission 670 nm | |
Maximum Excitation 650 nm | |
Unit Size 1 mg | |
Storage Instructions 2-8 °C | |
Sugar Specificity α2,6-sialylated LacNAc and α2,6-sialylated LacdiNAc | |
Usage Summary The recommended concentration range for use is 5-20 µg/ml. | |
Applications Immunofluorescence, Glycobiology | |
Concentration 1 mg active conjugate/ml | |
Conjugate Cy5 |
Sambucus Nigra Lectin (SNA, EBL), CY5
Sambucus nigra lectin, isolated from elderberry bark, binds preferentially to sialic acid attached to terminal galactose in α-2,6 and to a lesser degree, α-2,3 linkage. Binding is also inhibited to some extent by lactose or galactose. This lectin appears to bind sialic acid linked to N-acetylgalactosamine or galactose. SNA has been reported to inhibit cell-free protein synthesis.
Cy5 labeled Sambucus nigra lectin has an appropriate number of fluorochromes bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated fluorochromes.
• Excitation: 650 nm
• Emission: 670 nm
• Color: Far Red
$247.00
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Technical Information Accompanying each fluorescent lectin is an analysis data sheet summarizing the results of our quality control tests and providing pertinent information on the product. All of these reagents are supplied as solutions preserved with sodium azide. Elution: 500 mM lactose in buffered saline followed by 500 mM lactose in acetic acid |
