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Cy5 Biotin (CCT-1049)

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Description

Cy5 Biotin can be used for detecting and quantifying biotin binding sites of avidin, streptavidin or neutravidin. This reagent overcomes major shortcomings of commonly used Biotin-4-fluorescein – poor solubility in aqueous media and pH dependent absorbance and emission. Cy5 Biotin is a water soluble reagent and its red fluorescence is pH independent from pH 4 to pH 10. A flexible PEG3 spacer between biotin moiety and fluorescent tag minimize steric hindrance involved in binding to avidin, streptavidin or neutravidin.

Specifications

Unit Size1 mg, 5 mg
Abs/Em Maxima649/671 nm
Extinction Coefficient
250,000
Flow Cytometry Laser Line
633 or 635 nm
Microscopy Laser Line
633 or 635 nm
Spectrally Similar DyesAlexa Fluor® 647, CF™ 647 Dye, DyLight™649
Molecular weight1136.39
CASN/A
SolubilityWater, DMSO, DMF, MeOH
Purity>95% (HPLC)
AppearanceBlue solid
Storage Conditions-20°C. Desiccate
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy Cy

Selected References

  1. Schendel, L. C., et al. (2020). Single-Molecule Manipulation in Zero-Mode Waveguides. Small.16 (13), e1906740. [PubMed]
 

Applicable patents and legal notices are available at legal notices.

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