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Sulfo Cy5 Maleimide (FP-1676)

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Description

Sulfo Cy5 Maleimide is a water-soluble, bright, far-red-fluorescent dye with excitation ideally suited for the 633 nm or 647 nm laser lines. A significant advantage to using long wavelength dyes such as Sulfo Cy5, AZDye 647 or IR 650 dye over other fluorophores is the low autofluorescence of biological specimens in this region of the spectrum.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. Sulfo Cy5 conjugates are only recommended for detection of moderate-to-high abundance targets. For detection of low-abundance biological targets we recommend using AZDye 647 Maleimide or IR 647 Maleimide, which can attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityThiol (Cys)
Abs/Em Maxima648/671 nm
Extinction coefficient250,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 647, DyLight® 649,
Molecular weight872.26
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

Cy Cy

Protein Labeling Calculator

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