Carboxyrhodamine 110 DBCO is an azide reactive probe used for imaging azide-tagged biomolecules via a copper-free “click reaction”. DBCO moiety reacts with azides to form a stable triazole and does not require Cu-catalyst or elevated temperatures allowing detection of azide-tagged biomolecules under mild conditions. In application where the presence of copper is a concern Carboxyrhodamine110 DBCO is an ideal alternative to copper requiring fluorescent alkynes.
Carboxyrhodamine110, a non-sulfonated version of Alexa Fluor® 488, is a bright, and highly photostable, green-fluorescent probe optimally excited by the 488 nm laser line. Its absorption/emission spectra is a similar to those of DyLight® 488, Alexa Fluor® 488.
Carboxyrhodamine110 DBCO reagent is not suitable for staining intracellular components of fixed and permeabilized cells due to high backgrounds.
|Unit Size||1 mg, 5 mg, 25 mg|
|Abs/Em Maxima||501/523 nm|
|Flow Cytometry Laser Line|
|Microscopy Laser Line|
|Spectrally Similar Dyes||Fluorescein, Alexa Fluor® 488, DyLight® 488|
|Storage Conditions||-20°C. Desiccate|
|Shipping Conditions||Ambient temperature|
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