Normal Goat Serum Blocking Solution, 2.5%

S-1012-50

SKU	Unit Size	Price	Qty
S-1012-50	50 ml	$112.00 See our distributors

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Description

Specifications

Documents

Product FAQs

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Description

2.5% Normal Goat Serum can be used for blocking non-specific binding or as an antibody diluent.

Features:

Pooled samples collected from healthy adult animals
Heat-treated and centrifuged to remove precipitates and then filtered
Tested with appropriate antibody to check for possible cross-reactivities
Ready-to use 2.5% solution (v/v) in buffer containing 0.08% sodium azide
Same serum preparation that is included in the ImmPRESS^® Detection Systems

Specifications

More Information
Unit Size	50 ml
Applications	Immunohistochemistry / Immunocytochemistry, Immunofluorescence
Blocking Action	Non-Specific Protein Blocking
Format	Ready-to-Use

Documents

Product FAQs

I recently purchased Normal Goat Serum, Cat. No. S-1000, from Vector Laboratories for use as a general block in my tissue staining applications. What is the percentage of serum, and once diluted, how long can I store the working solution?

Normal Goat Serum, Cat. No. S-1000, is essentially spun whole blood, filtered, heat inactivated and preserved with the addition of sodium azide. The 20 ml provided therefore is all goat serum. For most IHC and IF tissue staining applications, an aliquot is taken and diluted in assay buffer such as PBS or TBS to 2-10% (v/v). This working solution can be stored in the fridge and used for up to one week. Beyond this time, there would be concern for possible microbial contamination that would affect performance.