NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.
Biotinylated Narcissus pseudonarcissus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
|Unit Size||2 mg|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology|
|Recommended Usage||For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer.|
|Recommended Storage||2-8 °C|
|Solution||10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide|
|Concentration||2 mg active conjugate/ml|
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
This lectin exists as a tetramer at neutral pH but below pH 5.0 and above pH 9.0, NPL dissociates into monomers.
This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative.
Inhibiting Sugar: 400 mM α-methylmannoside
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