Molecular Weight 59 | |
Extinction Coefficient 1.87 | |
Formulation 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide | |
Inhibiting or Eluting Sugar a-methyl-mannoside | |
Unit Size 2 mg | |
Storage Instructions 2-8 °C | |
Sugar Specificity Terminal Manα1−6 and terminal Manα1−3 | |
Usage Summary For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the below buffer. | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology | |
Concentration 2 mg active conjugate/ml | |
Conjugate Biotinylated |
Narcissus Pseudonarcissus (Daffodil) Lectin (NPL, NPA), Biotinylated
NPL, isolated from daffodil bulbs, has a specificity toward α-linked mannose, preferring polymannose structures containing (α-1,6) linkages. Binding to mannose polymers can occur via internal mannose residues and is not dependent on structural integrity of a non-reducing end sugar. NPL also binds some galactomannans, and differs in other binding characteristics from a related lectin, Galanthus nivalis lectin. Unlike Con A, LCA, or PSA, NPL does not bind glucose.
Biotinylated Narcissus pseudonarcissus lectin has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
$219.00
| SKU | Unit Size | Price |
|---|---|---|
Select a unit size:
How do I request a quote or bulk pricing?
Technical Information This lectin exists as a tetramer at neutral pH but below pH 5.0 and above pH 9.0, NPL dissociates into monomers. This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibiting Sugar: 400 mM α-methylmannoside |
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
