Molecular Weight 107 | |
Extinction Coefficient 1.51 | |
Inhibiting or Eluting Sugar L-Fucose | |
Unit Size 2 mg | |
Storage Instructions 2-8 °C; Store frozen for long term storage | |
Sugar Specificity α1,3-linked fucose | |
Usage Summary Reconstitute by adding 1ml water. The resulting solution will have the following composition: 10 mM HEPES, pH 7.5, 0.15 M NaCl, 0.08% sodium azide, 0.1 mM CaCl2.For most applications, we recommend a freshly prepared working solution of 5-20 µg/ml in the above buffer. | |
Applications Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology | |
Conjugate Biotinylated |
Lotus Tetragonolobus Lectin (LTL), Biotinylated
Lotus tetragonolobus lectin is a family of closely related glycoproteins that appear to have similar specificities toward α-linked L-fucose containing oligosaccharides. Although many of the binding properties of Lotus lectin are similar to those of Ulex europaeus lectin I, the binding affinities and some specificities for oligosaccharides are significantly different between these fucose-specific lectins.
Biotinylated Lotus tetragonolobus has an appropriate number of biotins bound to provide the optimum staining characteristics for this lectin. This conjugate is supplied essentially free of unconjugated biotins and is preserved with sodium azide.
$219.00
| SKU | Unit Size | Price |
|---|---|---|
Select a unit size:
How do I request a quote or bulk pricing?
Technical Information This biotinylated lectin is an ideal intermediate for examining glycoconjugates using the Biotin-Avidin/Streptavidin System. First the biotinylated lectin is added, followed by the VECTASTAIN ABC Reagent, Avidin D conjugate, or streptavidin derivative. Inhibiting/Eluting Sugar: 50 mM – 100 mM L-fucose |
Powered by Bioz © 2023
Product FAQs
I recently purchased a biotinylated lectin. The datasheet supplied with the lectin suggests including 0.1 mM Ca++as part of the recommended buffer to prepare a working solution. What should I specifically add, and why is this required?
From our experience we have found that some lectins require Ca++ to be present for optimal binding activity. We suggest using calcium chloride (CaCl2) to fortify working solutions and ensure a minimum level of Ca++ is met. This may be particularly pertinent if using phosphate based buffers as diluents and storage solutions.
