These antibodies to lectins are produced by immunizing goats with pure lectins. Following conventional purification steps, specific antibody is isolated by affinity chromatography on lectin-agarose columns. Each anti-lectin is supplied in buffered saline.
Biotinylated anti-lectins are optimally labeled with biotin.
|Unit Size||0.5 mg|
|Applications||Immunohistochemistry / Immunocytochemistry, Immunofluorescence, Blotting Applications, Elispot, ELISAs, Glycobiology|
|Concentration||1 mg active conjugate/ml|
|Recommended Storage||2-8 °C; Store frozen for long term storage|
|Solution||10 mM sodium phosphate, pH 7.8, 0.15 M NaCl, 0.08% sodium azide, 20 mg/ml sucrose|
|Recommended Usage||The recommended concentration range for use is 5-20 µg/ml. If this biotinylated antibody is to be used in tissues, which may contain cross-reacting endogenous immunoglobulins, dilution of this biotinylated antibody may be made in buffers containing 2% normal serum from the same species as the tissue.|
These highly purified specific antibodies provide excellent intermediate reagents for localizing lectin receptors in tissues and on cells or following lectin transport in neuronal tracing.
Biotinylated anti-lectins can be used as intermediates to localize lectin receptors in tissues or on cells, or to follow lectin migration in neuronal transport. In some applications, biotinylated anti-lectins used in conjunction with unlabeled lectins may provide greater sensitivity than using biotinylated lectins alone.
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