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AZDye 660 Maleimide (FP-1822)

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Description

AZDye 660 is a bright and photostable near-IR dye that is spectrally almost identical to Alexa Fluor® 660 and CF660® 680RD. AZDye 680 is often used for a bright and photostable far-red dye with excitation ideally suited to the 633 or 647 nm laser line. AZDye 660 ideally suited for the 633 nm laser line. This near-IR fluorescent dye is water soluble and pH-insensitive from pH 4 to pH 10. AZDye 660 molecules can be attached to proteins at high molar ratios without significant self-quenching, leading to brighter conjugates and more sensitive detection. The long wavelength emission allows for detection in complex samples with auto-fluorescent background signals.

Maleimide is the most popular sulfhydryl-reactive reactive group for conjugating the dye to a thiol group on a protein, oligonucleotide thiophosphate, or low molecular weight ligand. The maleimide group specifically and efficiently reacts with reduced thiols (sulfhydryl groups, –SH) at pH 6.5 to 7.5 to form stable thioether bonds.

Specifications

Unit Size1 mg, 5 mg, 25 mg, 100 mg
ReactivityThiol (Cys)
Abs/Em Maxima662/690 nm
Extinction coefficient250,000 cm-1M-1
SolubilityWater, DMSO, DMF
Spectrally similar dyesAlexa Fluor® 660, CF® 660
Molecular weight1111.25
Storage Conditions-20°C.
Shipping ConditionsAmbient temperature

Abs/Em Spectra

AF660

Protein Labeling Calculator

[protein-labeling-calculator]

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